Please, complete all fields.DATE OF REQUEST (yyyy/mm/dd)* Date Format: YYYY slash MM slash DD YOUR CONTACT INFORMATIONS*MrsMr.First and Last Name*Email* Phone Number*You are working in:*An academic laboratory (Public Laboratory)A private companyEntitled your laboratory or company*Entitled of your team or department*Scientific themes of your laboratory or company (maximum 4 keywords)*Complete address of your laboratory or company*No. and street:*Zip code:*City*Country*YOUR PROJECTSummary of your scientific project (2000 characters maximum, including space)* Tick the appropriate platform and service choice (several possible choices) ESTEAM PARIS-SUD Reprogramming and organoid engineering Platform - Research grade: Modeling of somatic and hereditary malignancies (solid tumor and hematology) (ESTeam Villejuif)ESTEAM PARIS-SUD Generation of human IPSC lines via reprogramming from various starting cells-research grade and characterizations (international standards: pluripotency assays, cell identity viability, sanitary tests) Generation of bioengineering of organoïds by 3D-culture methods from iPSC and progenitors (pathological models, toxicity and efficacy of advanced therapy medicinal products) Genome editing of IPSC lines and derived (Cells and organoids- pathological models) New cGMP Cell Factory Platform of IPSC and ATMPs bioproduction – clinical grade- for cellular therapy and immunotherapy (Cancers, Regenerative medicine) (ESTeam Evry) Plateform BPF de Bioproduction de cellules IPSC Advanced manufacturing of safe, allogeneic and qualified cGMP-IPSC from haplotyped donors, universal or super-donors with international standards. Bioproduction of qualified cGMP-IPSC and derived cells by automated close system culture- Flexible Scale-out/ Scale-up of cGMP iPSC Bank stock. Evaluation of iPSC-derived cell therapy products with suitability standards for analytic tests (genomic integrity assays, immunogenicity, tumorigenicity, potency assays evaluating functionality, mode of action) Technological supports, specific automated equipment, access to cGMP clean rooms for technological co-development programs in bioproduction, design of new therapeutic tools / models and analytical tests I-STEM Automated cell factory Platform of ESC/IPSC and derivatives- Research grade- for therapeutic molecular screening: Modeling of rare genetic diseases (Évry)I-STEM Large scale and automated production of human pluripotent cells (hESC/hiPSC research grade) from healthy and/or mutation-bearing subjects (expert in rare diseases) Large scale and automated production of large banks of progeny derived from human pluripotent cells (hES/hiPS research grade) from healthy and/or mutation-bearing subjects (RPE, MSC, Keratinocytes, NSC) High and/or medium throughput screening of molecules with therapeutic potential IGBMC High throughput molecular screening Platform for ESC/IPSC differentiation induction (Strasbourg)IGBMC Cell-based assay development for high throughput screening siRNA-based and small molecule screening (cell-based assays) CRISPR-Cas9 pooled lentiviral screening (knockout, activation, repression) on Cas9 cell lines CRISPR/Cas9 genome-wide screening method PRIMASTEM/SBRI Reprogramming and ESC derivation Platform of non-human primate for in vivo models (Lyon)PRIMASTEM/SBRI Production of cynomolgus macaque (Macaca fascicularis) embryos by in vitro fertilization Production of macaque monkey (Macaca mulatta or Macaca fascicularis) iPSC lines Production of transgenic macaques using lentiviral vectors Pre-clinical model: Monkey macaque cynomolgus (Macaca fascicularis) + autologous iPSC line (biological validation, toxicity / biodistribution) SAFE-IPS Reprogramming and organoid engineering Platform - Research grade: Modeling of aging diseases (Montpellier)SAFE-IPS Production and characterization of human iPSC lines Characterization of IPSC lines: International standards: pluripotency assays, geno-identity, viability, sanitary tests Set up differentiation Protocols Production and characterization of organoïds Equipment rental dedicated to cell culture such as the Ruskin hood FORMATION Organisation of practical technological training course (project hotel, sponsored workshop) Formation Pluripotent stem cells culture methods and characterization: - Non-human primates (macaque, marmoset) (PrimaStem) - Human iPSC (ESTeam and SAFE-IPSC). Production and characterization of differentiated cells from iPSC: embryoid body, cardiac cells, mesenchymal stem cells, neural stem cells, definitive endoderm, other) (SAFE-iPS, I-STEM) Cellular high throughput screening (IGBMC) PhoneThis field is for validation purposes and should be left unchanged.