The Institute for Research on Stem Cells and Brain in Lyon (SBRI) studies the development of neuronal circuits in normal and pathological conditions, in particular in Parkinson’s disease.

PrimaStem is a biotechnology platform on stem cells  funded by INSERM and INRA in the UMRS 846. The goal of PrimaStem within INGESTEM is to generate ES/ IPSC-based germline chimaeras in non-human primates, rabbits, and rodents for transgenic models.


  • Scientific Direction: Dr. Pierre Savatier
  • Technical manager: Dr. Marielle Afanassieff


  • Engineering non murine pluripotent stem cells.
  • Molecular mechanisms involved in self-renewal in the naive pluripotent state.
  • Animal transgenesis.

Involvement in INGESTEM:

  • Production of somatic and germline chimeras in different species including rabbits and non-human primates from ES and iPS cell lines.
  • Generation of pluripotent stem cell lines that self-renew in naive pluripotent state (study of early development)
  • Construction of IPSC with gene reporter for monitoring the behavior of grafted cells in vivo (safety and efficacy of cell therapies).

Major publications:

  • Reinforcement of STAT3 activity reprogrammed human embryonic stem cells to pluripotency-like naive. Chen H, Aksoy I Gonnot F, Osteil P Aubry M, Hamela C Rognard C. Hochard A Neighbor S, Fontaine E, Mure million Afanassieff million Cleroux E, Guibert S, Chen J, Vallot C Acloque H , C. Genthon, Donnadieu C. De Vos J, Sanlaville D Guerin JF, Weber M, Stanton LW, C Rougeulle Bread B, PY Bourillot, Savatier P. Nat Commun. 2015 May 13; 6: 7095.
  • Generation of Embryonic Stem Cells in Rabbits. Afanassieff M, P Osteil, Savatier P. Methods Mol Biol. 12 March 2015.
    Klf4 and KLF5 differentially inhibit mesoderm and endoderm differentiation in embryonic stem cells. Aksoy I Giudice V, Delahaye E, Wianny F Aubry M, Mure M, Chen J, Jauch R, Bogu GK, Nolden T Himmelbauer H, Xavier Doss M, Sachinidis A, Schulz H, Hummel O, Martinelli P, Hübner N, Stanton LW, Real FX, Bourillot PY, Savatier P. Nat Commun. 2014 Apr 28; 5: 3719.
  • Induced pluripotent stem cells derived from rabbits Some exhibit characteristics of pluripotency naive. Osteil P Tapponnier Y, Markossian S, Bucket M Schmaltz-Panel B, Jouneau L, Cabau C. Joly T, Blachere T Gócza E, Bernat A, Yerle million Acloque H, Hidot S, Bosze Z Duranthon V Savatier P, M. Biol Afanassieff Open. 10 May 2013; 2 (6): 613-28.
  • Transgenic rhesus monkeys produced by gene transfer into early cleavage embryos using internship has simian immunodeficiency virus-based vector. Niu Y., Y. Yu, A. Bernat, S. Yang, X. He, X. Guo, D. Chen, Y. Chen, Ji S., W. If, T. Tan, Q. Wei, Wang H ., L. Shi, Afanassieff M., P. * Savatier, Q. Zhou *, and * Ji W. (2010) Proc Natl Acad Sci USA. 107: 17663-7 [* co-senior investigators].
  • A short G1 is an intrinsic determinant of artless embryonic stem cell pluripotency. Coronado D., M. Godet, Bourillot P.-Y., Tapponnier Y., A. Bernat, Mr. Petit, Mr. Afanassieff, Markossian S., A. Malashicheva, Iacone R., K. Anastassiadis, and Savatier P . (2013). Stem Cell Res. 10: 118-31.
  • Molecular and functional features demarcate embryonic and induced pluripotent stem cells in rabbits. Osteil P., Y. Tapponnier, Markossian S., M. Godet, Schmaltz-Panel B., L. Jouneau, Cabau, C. Joly, Blachere T., Gozca, E., A. Bernat, Yerle M., Acloque H., S. Hidot, Bözse, Z., V. Duranthon, Savatier P. *, and * Afanassieff M. (2013). Open Biology. 2: 613-28. * Co-senior authors.

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